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Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc02418a
| Content Provider | Semantic Scholar |
|---|---|
| Author | Walsh, Sarah El-Sagheer, Afaf Helmy Brown, Tom |
| Copyright Year | 2018 |
| Abstract | The instability of DNA triplexes particularly at neutral pH and above severely limits their applications. Here, we demonstrate that the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs) resolves this problem. The stabilising effects are additive; multiple TO units produce nanomolar duplex binding and triplex stability can surpass that of the underlying duplex. In one example, a TFO containing three TO units increased the triplex melting temperature at pH 7 by a remarkable 50 °C relative to the unmodified triplex. Notably, TO intercalation promotes TFO binding to target sequences other than pure polypurine tracts by the use of 5-(1-propynyl)cytosine (pC) against C:G inversions. By overcoming the instability of triplexes across a broad range of pH and sequence contexts, these very simple 'TOTFO' probes could expand triplex applications into many areas including diagnostics and cell imaging. |
| Starting Page | 7681 |
| Ending Page | 7687 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/c8sc02418a |
| PubMed reference number | 30393529 |
| Journal | Medline |
| Volume Number | 9 |
| Alternate Webpage(s) | https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7c/de/SC-009-C8SC02418A.PMC6182420.pdf |
| Alternate Webpage(s) | https://doi.org/10.1039/c8sc02418a |
| Journal | Chemical science |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
