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Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat)
| Content Provider | Semantic Scholar |
|---|---|
| Author | Walsh, Catherine Joan Leggett, Stephanie R. Strohbehn, Kathryn Pierce, Richard Holton Sleasman, John William |
| Copyright Year | 2008 |
| Abstract | Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The objective of this research was to investigate potential immunotoxic effects of brevetoxins using a leukemic T cell line (Jurkat) as an in vitro model system. Viability, cell proliferation, and apoptosis assays were conducted using brevetoxin congeners PbTx-2, PbTx-3, and PbTx-6. The effects of in vitro brevetoxin exposure on cell viability and cellular metabolism or proliferation were determined using trypan blue and MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan), respectively. Using MTT, cellular metabolic activity was decreased in Jurkat cells exposed to 5 - 10 microg/ml PbTx-2 or PbTx-6. After 3 h, no significant effects on cell viability were observed with any toxin congener in concentrations up to 10 microg/ml. Viability decreased dramatically after 24 h in cells treated with PbTx-2 or -6. Apoptosis, as measured by caspase-3 activity, was significantly increased in cells exposed to PbTx-2 or PbTx-6. In summary, brevetoxin congeners varied in effects on Jurkat cells, with PbTx-2 and PbTx-6 eliciting greater cellular effects compared to PbTx-3. |
| Starting Page | 291 |
| Ending Page | 307 |
| Page Count | 17 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 18728729 |
| DOI | 10.3390/md20080014 |
| Journal | Marine drugs |
| Volume Number | 6 |
| Alternate Webpage(s) | http://www.mdpi.org/marinedrugs/papers/md6020291.pdf |
| Alternate Webpage(s) | http://www.mdpi.com/1660-3397/6/2/291/pdf/ |
| Alternate Webpage(s) | http://www.mdpi.com/1660-3397/6/2/291/pdf |
| Alternate Webpage(s) | https://doi.org/10.3390/md20080014 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Cell Proliferation Cell Survival Cellular Phone Dinophyceae Karenia brevis Leukemia, B-Cell Metabolic Process, Cellular Phycodnaviridae Ptychodiscus brevis T2 toxin Red Tide Tritium Trypan Blue brevetoxin caspase-3 activity monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
