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Evaluation of Different Serological Tests to Study Seroprevalence of Brucellosis in Buffaloes
| Content Provider | Semantic Scholar |
|---|---|
| Author | Soni, Mamta |
| Copyright Year | 2016 |
| Abstract | The present study was under taken to detect the Brucella antibodies in serum as well as in milk of buffaloes of Kota region of Rajasthan. The AB ELISA was used for detection of Brucella antibodies in conjunction with RBPT, STAT, HIT, MET, AGID and MRT to detect their efficacy as compared to AB ELISA. The overall seroprevalence of was found as; AB ELISA (37.84%), RBPT (35.09%), STAT (29.59%), HIT (27.52%), MET (26.38%) and AGID (13.99%) in total 436 buffalo serum samples and MRT (31.75%) in 63 milk samples. Highest seroprevalence was found in Chhabra region in all tests whereas lowest seroprevalence was found in Dag region by all the tests except HIT and MET which showed lowest prevalence in district Bundi. Higher number of females (38.46%) showed seropositivity for brucellosis as compared to males (30.30%) in AB ELISA. In the present investigation, the relative sensitivity value was highest with RBPT followed by MRT, STAT, HIT, MET and lowest with AGID. The relative specificity values were highest with AGID followed by MET, HIT, STAT, MRT and lowest with RBPT. The relative concordance values were highest with MET followed by HIT, STAT, RBPT, MRT and lowest with AGID. This study reveals prevalence of Brucella infection in buffaloes of Kota division and necessitates early detection and proper monitoring of brucellosis at State level to eradicate the brucellosis. HkSlksa esa czqlsyksfll ds lhjeh; izk;ksHkko ds v/;;u gsrq fofHkUu lhjeh; ijh{k.kksa dk ewY;kadu LukrdksÙkj 'kks/k xzaFk Ik’kq lw{e thofoKku] Ik'kq fpfdRlk ,oa Ik'kq foKku egkfo|ky;] jktLFkku Ik'kq fpfdRlk ,oa Ik'kq foKku fo’ofo|ky;] chdkusj & 334001 izLrqrd=hZ % eerk lksuh mins"Vk % MkWch-,uJ`axh vuq{ksi.k bl v/;;u ds vUrxZr dksVk laHkkx dh HkSlksa ds lhje o nqX/k esa czqlsyk izfrfi.M+ks dh [kkst dh xbZA ftlesa ,Utkbe fyadM bE;wuks lksjcsUV ,sls ijh{k.k o vU; ijh{k.k tSls vkj-ch-ih-Vh-] ,l-Vh-,-Vh-] ,p-vkbZ-Vh-] ,e-bZ-Vh-] ,-th-vkbZ-Mho ,evkj-Vhijh{k.kksa ls czqlsyk izfrfi.M+ks dh [kkst dh xbZ vkSj lHkh ijh{k.kksa dh ,-chb-,y-vkbZ-,l-,dh rqyuk esa dk;Z{kerk dh [kkst Hkh dh xbZA lexz lhjeh; izk;ksHkko fuEu Øe esa ik;k x;kA ,-chb-,y-vkbZ-,l-,(37.84%), vkj-ch-ih-Vh(35.09%), ,l-Vh-,-Vh(29.59%), ,p-vkbZ-Vh(27.52%), ,e-bZ-Vh(26.38%) ,oa ,-th-vkbZ-Mh(13.99%) dqy 436 lhjeh; uequksa esa o ,e-vkj-Vh(31.75%) 63 nqX/k uequksa esa ik;k x;kA lHkh ijh{k.kksa esa NcM+k {ks= esa vf/kdre lhjeh; izk;ksHkko ik;k x;kA tcfd ,p-vkbZ-Vho ,e-bZ-Vhds vfrfjDr lHkh ijh{k.kksa esa fuEure izk;ksHkko Mx {ks= esa ik;k x;kA ,p-vkbZ-Vho ,e-bZ-Vhesa fuEure lhjeh; izk;ksHkko cwanh {ks= esa ik;k x;kA ,-chb-,y-vkbZ-,l-,esa uj dh vis{kk T;knk la[;k esa eknkvksa esa czlsyksfll dk lhjeh; izk;ksHkko ik;k x;kA bl v/;;u esa vf/kdre lkis{k laosnu’khyrk fuEu Øe esa ikbZ xbZ & vkj-ch-ih-Vh-] ,e-vkj-Vh-] ,l-Vh-,-Vh-] ,p-vkbZ-Vh-] ,e-bZ-Vho fuEure lkis{k laosnu’khyrk ,-th-vkbZ-Mhesa ikbZ xbZA lkis{k fof’k"Vrk fuEu Øe esa ikbZ xbZ & ,-th-vkbZ-Mh,e-bZ-Vh-] ,p-vkbZ-Vh-] ,l-Vh-,-Vh-] ,e-vkj-Vho fuEure lkis{k fof’k"Vrk vkj-ch-ih-Vhesa ikbZ xbZA leLr vUOk; fuEu Øe esa ik;k x;k & ,e-bZ-Vh-] ,p-vkbZ-Vh-] ,l-Vh-,-Vh-] vkj-ch-ih-Vh-] ,e-vkj-Vho fuEure leLr vUo; ,-th-vkbZ-Mhesa ik;k x;kA ACKNOWLEDGEMENTS I consider myself fortunate to have worked under the supervision and guidance of Dr. B. N. Shringi, Assistant Professor, Department of Veterinary Microbiology and Biotechnology, R.U.V.A.S., Bikaner, who gave his invaluable suggestions and scholarly guidance throughout my study and research work. I express my heart-felt gratitude to Minor Advisor Dr. S. Maherchandani Assistant Professor, Department of Veterinary Microbiology and Biotechnology, R.U.V.A.S., Bikaner for his consistent and invaluable inspirations, prolific and introspective guidance with constructive suggestions and active persuasion throughout the course of my study. I extend sincere thanks to the members of my advisory committee Dr. (Mrs.) Indu Vyas, Assistant Professor, Department of Veterinary Pathology and Dr. (Mrs.) Nalini Kataria, Assistant professor, Department of Veterinary Physiology, R.U.V.A.S., Bikaner for their constant help and support throughout the course of study. I am highly grateful to reverend Dr. S. K. Kashyap, Professor and Head, Department of Veterinary Microbiology and Biotechnology for his emphatic help, intellectual stimulation and critical amendments made during the entire course of post graduation. I also owe my sincere thanks to Dr. S. S. Soni, Associate Professor, Department of Veterinary Microbiology and Biotechnology, Dr. A. K. Kataria, Assistant Professor, Department of Veterinary Microbiology and Biotechnology, R.U.V.A.S., Bikaner for their ever willing help and goodwill. I am thankful to Dr. S.B.S, Yadav, Dean, C.V.A.S, Bikaner for his generous attitude in providing necessary facilities to carry out the research work. The constant assistance provided by technical staff, Shri Suresh Sharma, Shri Ravi Purohit, Shri Neelesh and Deepak and nontechnical staff, Shri Moda Ram, late Shri Mangtu Ram, Vimla Bai, Shri Ramesh and Rukmani Bai is appreciated. I am highly thankful to my respected seniors Balram, Ritu, Padma and Bharti , my dear colleagues Priya, Sarjana, Anita, Manju, Varsha, Abhinav and Naresh and my dear juniors Dharam Gopal, Ruchi, Sneha, Vikas and Bhavana for their whole hearted co-operation during the course of study. My reverend parents, my brother and sister, and all my near and dear ones deserve not just thanks but much more than what I can weight in words. And I would not be what I am, if I do not thank that one heavenly soul, the Almighty for His untold blessings. Place: Bikaner Date: Mamta Soni |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://krishikosh.egranth.ac.in/bitstream/1/96830/1/Mamta%20Soni.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
