NDLI: Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants.

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Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants.

Content Provider	Europe PMC
Author	Sadhu, Dashrath B. Panchasara, H. H. Chauhan, H. C. Sutariya, D. R. Parmar, V. L. Prajapati, H. B.
Copyright Year	2015
Abstract	Aim:The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat.Materials and Methods:Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serological tests viz., Rose bengal plate test (RBPT), Standard tube agglutination test (STAT), and Indirect Enzyme-linked immunosorbent assay (I-ELISA).Results:The seroprevalence of brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by RBPT, STAT, and I-ELISA, respectively. The seroprevalence of brucellosis was found to be higher in sheep than goats. The sensitivity of RBPT was found slight more than STAT, but the specificity of both tests was same. In this study, the overall agreement of RBPT and STAT with I-ELISA was found 92.50% and 92.30% in small ruminants, respectively.Conclusion:I-ELISA was a better serological test as compared to RBPT and STAT in the sense of sensitivity, specificity, and rapidity and it could be advocated for screening of brucellosis in sheep and goats.
ISSN	09728988
Journal	Veterinary World
Volume Number	8
PubMed Central reference number	PMC4774713
Issue Number	5
PubMed reference number	27047135
e-ISSN	22310916
DOI	10.14202/vetworld.2015.561-566
Language	English
Publisher	Veterinary World
Publisher Date	2015-05-04
Publisher Place	India
Access Restriction	Open
Rights License	This article is an open access article licensed under the terms of the Creative Commons Attributin License (http://creative commons.org/licenses/by/2.0) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited. Copyright: © The authors.
Subject Keyword	brucellosis seroprevalence serological test small ruminant
Content Type	Text
Resource Type	Article
Subject	Veterinary

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