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Quantitative autoradiographic localization of [125I-TYR8]bradykinin receptor binding sites in the rat spinal cord: Effects of neonatal capsaicin, noradrenergic deafferentation, dorsal rhizotomy and peripheral axotomy
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lopes, Patrice Kar, Satyabrata Chrétien, Louise Regoli, Domenico Couture, Réjean |
| Copyright Year | 1995 |
| Abstract | In vitro receptor autoradiography was used to localize, quantify and characterize [125I-Tyr8]bradykinin binding sites in all major spinal cord segments of normal rats and animals subjected to various chemical treatments and surgical lesions. [125I-Tyr8]bradykinin specific binding sites were predominantly located to superficial laminae of the rat dorsal horn, with the substantia gelatinosa showing the highest density of labelling (values ranging from 3.1 fmol/mg tissue in cervical to 4.5 fmol/mg tissue in lumbar segments). A moderate density (1.8-3.0 fmol/mg tissue) of specific binding was observed in lamina III, whereas in other areas, i.e. laminae I and IV-X, lower amounts of labelling were detected. Within the superficial laminae of the dorsal horn, [125I-Tyr8]bradykinin binding was largely distributed over the neurophil with some perikarya showing concentrations of labelling. In contrast, the ventral horn showed a rather homogeneous distribution of [125I-Tyr8]bradykinin binding over the neuropil, with silver grain alignments surrounding motoneuron perikaryas and proximal processes. Bradykinin, [Tyr8]bradykinin and B2 receptor antagonists (D-Arg[Hyp3,Thi5,D-Tic7,Oic8]bradykinin (Hoe 140), D-Arg[Tyr3,D-Phe7,Leu8]bradykinin, D-Arg[Hyp3, Leu8]bradykinin, D-Arg[Hyp2, Thi5,8,-Phe7]bradykinin D-Arg[Hyp3, D-Phe7, Leu8]bradykinin, Tyr0, D-Arg[Hyp3, D-Phe7, Leu8]bradykinin inhibited [125I-Tyr8]-bradykinin binding with very high subnanomolar affinities, while the B1 receptor agonist (Tyr0,des-Arg10-kallidin) and antagonist ([Leu8]-des-Arg9-bradykinin) did not significantly affect [125I-Tyr8]bradykinin binding at up to micromolar concentrations. Two weeks after unilateral lumbar dorsal rhizotomy (L1-L6) or peripheral lesions of the sciatic nerve, significant decreases ( +/- 50%) in [125I-Tyr8]bradykinin binding sites were found in ipsilateral laminae I-III of lumbar spinal cord.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Starting Page | 867 |
| Ending Page | 881 |
| Page Count | 15 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/0306-4522(95)00161-B |
| PubMed reference number | 8577380 |
| Journal | Medline |
| Volume Number | 68 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/030645229500161B |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/030645229500161B?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/0306-4522%2895%2900161-B |
| Journal | Neuroscience |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
