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Lipolysis and apoptosis of adipocytes induced by neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides in obese rats.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gong, Hai-Xia Guo, Xi-Rong Liu, Qian-Qi Chen, Rong-Hua |
| Copyright Year | 2003 |
| Abstract | AIM To investigate the influence of central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides (ODN) on the body weight and fat pads of high-energy diet-induced obese rats, and the effects on white adipocyte lipolysis and apoptosis. METHODS Y5 receptor antisense, sense, mismatched oligodeoxynucleotides (ODN) or vehicle were intracerebroventricularly injected, and average adipocyte area was calculated. DNA ladders were measured to evaluate adipocyte apoptosis, and RT-PCR was used to analyze the expression of bcl-2 and bax gene. RESULTS (1) Central administration of Y5 receptor antisense ODN significantly decreased body weight, fat pads, and average adipocyte area. (2) DNA fragmentation was presented after electrophoresis at both epididymal and retroperitoneal adipose tissue. (3) The expression of bcl-2 gene was downregulated, while the expression of bax was upregulated. CONCLUSION Lipolysis and adipocyte apoptosis may be important reasons for Y5 receptor antisense therapy. |
| File Format | PDF HTM / HTML |
| PubMed reference number | 12791184 |
| Journal | Medline |
| Volume Number | 24 |
| Issue Number | 6 |
| Alternate Webpage(s) | http://www.chinaphar.com/article/download/9159/9822 |
| Journal | Acta pharmacologica Sinica |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
