NDLI: Identificação de Cronobacter spp. em queijos e perfil de suscetibilidade antimicrobiana

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Identificação de Cronobacter spp. em queijos e perfil de suscetibilidade antimicrobiana

Content Provider	Semantic Scholar
Author	Brandão, Marcelo Luiz Lima Meier, Gisele Olivieri Soares Carvalho, Carla Trece Silva, Débora Alves Da Umeda, Natália Scudeller Medeiros, Valéria De Mello Rosas, Carla De Oliveira Lopes, Silvia Maria Dos Reis
Copyright Year	2016
Abstract	Cronobacter spp. is an opportunistic pathogen that may cause infections in individuals of any age, and the highest incidence occurs in neonates, immunocompromised patients and elderly persons. This study investigated Cronobacter spp. occurrence in 90 cheese samples (30 “Minas Frescal” type cheeses, 30 “Prato” type and 30 sliced “Prato” type). The isolated species were identified and the antimicrobial susceptibility profile of the isolated strains was evaluated. The microbiological assay was performed with pre-enrichment in buffered peptone water, and selective-enrichment in modified lauryl sulphate tryptose broth containing vancomycin. Isolation and identification were done in Enterobacter sakazakii Isolation Agar and in Vitek 2.0, respectively. The species identification was performed by multiple PCR targeting cgaA gene. Antibiogram was done using agar diffusion method (Kirby-Bauer). Cronobacter spp. was isolated from one (1.1 %) sample of “Minas Frescal” type cheese, identified as C. sakazakii which was sensitive to all of tested antimicrobials. Cronobacter spp. does not represent a risk to the individuals health by consuming cheeses made from pasteurized milk. However, the presence of Cronobacter spp. in one sample of analyzed cheese indicates failures in their production, reinforcing the need for following the Good Manufacturing Practices.
Starting Page	1
Ending Page	6
Page Count	6
File Format	PDF HTM / HTML
Volume Number	75
Alternate Webpage(s)	https://www.arca.fiocruz.br/bitstream/icict/18186/2/Rev_Inst_Adolfo_Lutz_75_1697.pdf
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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