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XPD Functions as a Tumor Suppressor and Dysregulates Autophagy in Cultured HepG2 Cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zheng, Jian-feng Li, Lin-lin Lu, Juan Juan Yan, Kun Guo, Wu-hua Zhang, Ji-xiang |
| Copyright Year | 2015 |
| Abstract | BACKGROUND Recent clinical studies have linked polymorphisms in the xeroderma pigmentosum group D (XPD) gene, a key repair gene involved in nucleotide excision repair, to increased risk of hepatocellular carcinoma (HCC). However, the cellular effects of XPD expression in cultured HCC cells remain largely uncharacterized. Therefore, the aim of this study was to characterize the in vitro cellular effects of XPD expression on the HCC cell line HepG2. MATERIAL AND METHODS HepG2 cells were transfected as follows to create four experimental groups: pEGFP-N2/XPD plasmid (XPD) group, EGFP-N2 plasmid (N2) control group, lipofectamine™ 2000 (lipid) control group, and non-transfected (CON) control group. An MTT cell proliferation assay, Annexin V-APC apoptosis assay, colony formation assay, scratch wound migration assay, Transwell migration assay, and Western blotting of the autophagic proteins LC3 and p62 were conducted. RESULTS XPD expression significantly inhibited HepG2 cell proliferation (p<0.05), significantly promoted HepG2 cell apoptosis (p<0.05), significantly inhibited HepG2 colony formation (p<0.05), significantly decreased HepG2 cells' migratory ability (p<0.05), and significantly lowered HepG2 cells' invasive capacity (p<0.05). Western blotting showed that XPD expression significantly increased LC3 expression (p<0.05) and significantly reduced p62 expression (p<0.05). CONCLUSIONS XPD expression serves as a tumor suppressor and dysregulates autophagic protein degradation in HepG2 cells in vitro. Further in vivo pre-clinical studies and clinical trials are needed to validate XPD's potential as a tumor-suppressive gene therapy. |
| Starting Page | 1562 |
| Ending Page | 1568 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 26031757v1 |
| Alternate Webpage(s) | https://doi.org/10.12659/MSM.894303 |
| DOI | 10.12659/msm.894303 |
| Journal | Medical science monitor : international medical journal of experimental and clinical research |
| Volume Number | 21 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Annexins Apoptosis Cell Migration Assays Cell Proliferation Conflict (Psychology) Data Collection Genes, vif Hep G2 Cells Lipofectamine Liver carcinoma Manuscripts Neoplasms Nephroblastoma Nucleotide Excision Repair Tumor Suppressor Genes Western Blotting Xeroderma Pigmentosum Xeroderma Pigmentosum, Complementation Group D monooxyethylene trimethylolpropane tristearate tumor suppressor activity |
| Content Type | Text |
| Resource Type | Article |
