NDLI: Linkage and Allostery in snRNP Protein/RNA Complexes

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Linkage and Allostery in snRNP Protein/RNA Complexes

Content Provider	Semantic Scholar
Author	Williams, Sandra Gisela Hall, Kathleen B.
Copyright Year	2014
Abstract	Drosophila SNF is a member of the U1A/U2B″/SNF protein family that is found in U1 and U2 snRNPs, where it binds to Stemloop II and Stemloop IV of U1 and U2 snRNA, respectively. SNF also binds to the U2A' protein, but only in the U2 snRNP. Although previous reports have implicated U2A' as a necessary auxiliary protein for the binding of SNF to Stemloop IV, there are no mechanisms that explain the partitioning of U2A' to the U2 snRNP and its absence from the U1 snRNP. Using in vitro RNA binding isotherms and isothermal titration calorimetry, the thermodynamics of SNF/RNA/U2A' ternary complex formation have now been characterized. There is a very large binding cooperativity unique to Stemloop IV that favors formation of the SLIV/SNF/U2A' complex. The binding cooperativity, or heterotropic linkage, is interpreted with respect to linked conformational equilibria of both SNF and its RNA ligand and so represents an example of protein-RNA allostery.
Starting Page	3529
Ending Page	3539
Page Count	11
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ftp.ncbi.nlm.nih.gov/pub/pmc/20/c8/bi500192a.PMC4059526.pdf
Alternate Webpage(s)	https://pdfs.semanticscholar.org/3c2b/cded5b5c81ad5884afa819b312779e6b2b03.pdf
PubMed reference number	24849693v1
Alternate Webpage(s)	https://doi.org/10.1021/bi500192a
DOI	10.1021/bi500192a
Journal	Biochemistry
Volume Number	53
Language	English
Access Restriction	Open
Subject Keyword	Calorimetry Ligands Protein Family RNA Binding RNU2-1 gene Ribonucleoprotein, U1 Small Nuclear Ribonucleoprotein, U2 Small Nuclear SNRPA gene Small Nuclear RNA Thermodynamics Titration Method U2 small nuclear ribonucleoprotein Ab.IgG:PrThr:Pt:Ser:Ord genetic linkage stage IV childhood liver cancer
Content Type	Text
Resource Type	Article

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