Loading...
Please wait, while we are loading the content...
Similar Documents
U1 small nuclear RNP from Trypanosoma brucei: a minimal U1 snRNA with unusual protein components
| Content Provider | Semantic Scholar |
|---|---|
| Author | Pálfi, Zsófia Schimanski, Bernd Günzl, Arthur Luecke, Stephan Bindereif, Albrecht |
| Copyright Year | 2005 |
| Abstract | Processing of primary transcripts in trypanosomes requires trans splicing and polyadenylation, and at least for the poly(A) polymerase gene, also internal cis splicing. The trypanosome U1 snRNA, which is most likely a cis-splicing specific component, is unusually short and has a relatively simple secondary structure. Here, we report the identification of three specific protein components of the Trypanosoma brucei U1 snRNP, based on mass spectrometry and confirmed by in vivo epitope tagging and in vitro RNA binding. Both T.brucei U1-70K and U1C are only distantly related to known counterparts from other eukaryotes. The T.brucei U1-70K protein represents a minimal version of 70K, recognizing the first loop sequence of U1 snRNA with the same specificity as the mammalian protein. The trypanosome U1C-like protein interacts with 70K directly and binds the 5' terminal sequence of U1 snRNA. Surprisingly, instead of U1A we have identified a novel U1 snRNP-specific protein, TbU1-24K. U1-24K lacks a known RNA-binding motif and integrates in the U1 snRNP via interaction with U1-70K. These data result in a model of the trypanosome U1 snRNP, which deviates substantially from our classical view of the U1 particle and may reflect the special requirements for splicing of a small set of cis-introns in trypanosomes. |
| Starting Page | 2493 |
| Ending Page | 2503 |
| Page Count | 11 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 15863726v1 |
| Volume Number | 33 |
| Journal | Nucleic acids research |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/33/8/10.1093_nar_gki548/2/gki548.pdf?Expires=1492647083&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=emqyuGAx3i3OHK~SKlJreOXWJ62NjyNrnIVS216zMOXWO~ZEpvMBi5mYGcSTqu3rOdnzd~zMP3gdStCY7KD2kk8GBnBjuH5ekjvv9qVzYnHPapSAEAqS10YuOS-QBfR3vd-IEyEVo-RQT4-txXdtXaExSY6-RVzRs6FEdxEFOncU7~zoPU6oOwFQf4EEQOBGqRiF8fPnoNWoW8lKKejYpaIZt~0yiayc5uIzRAQ2~F6fC2bmdB0fzr20ds1niNWXkSjCQFYayCYIDzKLrdVEaJ1i2EfxxAHInb0IZ8orom8-JcYWzBMrOTPOyupGXmbiXaFZKDUqS-UVAowaAxup5g__ |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Introns Mammals Polyadenylation Protein Domain RNA Binding RNA Splicing Ribonucleoprotein, U1 Small Nuclear SNRPA gene Small Nuclear RNA Small Nuclear Ribonucleoproteins Spectrometry Trans-Splicing Transcript Trypanosoma U1 small nuclear ribonucleoprotein A Ab:PrThr:Pt:Ser:Ord |
| Content Type | Text |
| Resource Type | Article |
