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Extracellular matrix proteins as early markers in diabetic nephropathy.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jaeckle-Meyer, Irmtrud Szukics, B. Neubauer, Katrin Metze, V. Petzoldt, R. L. Stolte, Hilmar |
| Copyright Year | 1995 |
| Abstract | Basement membrane thickening and mesangial expansion characterize the renal involvement in diabetes mellitus and precede any symptoms of renal dysfunction, e.g., albuminuria and changes in glomerular filtration rate. Since the morphological changes can only be diagnosed by biopsy, this study was designed to investigate whether the urinary excretion of renal extracellular matrix proteins might reflect the morphological alterations. To specify the extent of renal involvement in diabetes, the patients, type I as well as type II diabetics, were classified according to their urinary albumin excretion: normal albumin excretion below 30 micrograms/min, microalbuminuria from 30 to 300 micrograms/min, and overt albuminuria above 200 micrograms/min. Laminin, collagen IV, and fibronectin, all intrinsic components of the renal extracellular matrix, were determined in serum and urine by radioimmunoassay or enzyme-linked-immunosorbent-assay, respectively. The results are given as median values (mean). Additionally, the urinary fragment pattern of fibronectin was analysed qualitatively by immunoblotting. Laminin concentrations in serum and in urine did not change in diabetics. Collagen IV decreased in serum of patients with increased albumin excretion (controls: mean = 255 micrograms/l, normoalbuminuric patients: mean = 56 micrograms/l, microalbuminuric patients: mean = 52 micrograms/l, and patients with overt albuminuria: mean = 70 micrograms/l; alpha < 0.01) and increased in urine (controls, normoalbuminuric and microalbuminuric patients: not detectable, patients with overt albuminuria: mean = 5 ng/12 h; apha < 0.001).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Starting Page | 46 |
| Ending Page | 57 |
| Page Count | 12 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://edoc.hu-berlin.de/bitstream/handle/18452/13959/cclm.1995.33.4.211.pdf?isAllowed=y&sequence=1 |
| PubMed reference number | 7626693v1 |
| Volume Number | 33 |
| Issue Number | 4 |
| Journal | European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
