NDLI: Bruton tyrosine kinase (Btk) suppresses osteoblastic differentiation

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Bruton tyrosine kinase (Btk) suppresses osteoblastic differentiation

Content Provider	Semantic Scholar
Author	Kaneshiro, Shoichi Ebina, Kosuke Shi, Kenrin Yoshida, Kiyoshi Otsuki, Dai Yoshikawa, Hideki Higuchi, Chikahisa
Copyright Year	2014
Abstract	The Tec family of nonreceptor tyrosine kinases has been shown to play a key role in inflammation and bone destruction. Bruton tyrosine kinase (Btk) has been the most widely studied because of its critical role in B cells. Furthermore, recent evidence has demonstrated that blocking Btk signaling is effective in ameliorating lymphoma progression and experimental arthritis. The role of Btk in osteoblastic differentiation has not been well elucidated. In this study, we demonstrated the role of Btk in osteoblastic differentiation and investigated the effects of a Btk inhibitor on osteoblastic differentiation in mouse preosteoblastic MC3T3-E1 cells, primary calvarial osteoblasts, and bone marrow stromal ST2 cells. Btk expression was detected in all three cell lines. Btk inhibition stimulated mRNA expression of osteoblastic markers (alkaline phosphatase, osteocalcin, and osterix) and promoted mineralization of the extracellular matrix. In addition, Btk knockdown caused increased mRNA expression of osteoblastic markers. Furthermore, Btk inhibition suppressed the phosphorylation of mitogen-activated protein kinase (MAPK), nuclear factor kappa B (NFκB), and protein kinase Cα (PKCα). Our results indicate that Btk may regulate osteoblastic differentiation through the MAPK, NFκB, and PKCα signaling pathways.
Starting Page	486
Ending Page	495
Page Count	10
File Format	PDF HTM / HTML
DOI	10.1007/s00774-014-0612-8
PubMed reference number	25230818
Journal	Medline
Volume Number	33
Alternate Webpage(s)	http://file2.selleckchem.com/citations/PCI-32765-J-Bone-Miner-Metab-20140925.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s00774-014-0612-8
Journal	Journal of Bone and Mineral Metabolism
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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