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Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Planat-Benard, Valérie Ménard, Cédric André, Mireille Pucéat, Michel Pérez, Angel García-Verdugo, - Pénicaud, Luc Casteilla, Louis |
| Copyright Year | 2004 |
| Abstract | Cardiomyocyte regeneration is limited in adult life. Thus, the identification of a putative source of cardiomyocyte progenitors is of great interest to provide a usable model in vitro and new perspective in regenerative therapy. As adipose tissues were recently demonstrated to contain pluripotent stem cells, the emergence of cardiomyocyte phenotype from adipose-derived cells was investigated. We demonstrated that rare beating cells with cardiomyocyte features could be identified after culture of adipose stroma cells without addition of 5-azacytidine. The cardiomyocyte phenotype was first identified by morphological observation, confirmed with expression of specific cardiac markers, immunocytochemistry staining, and ultrastructural analysis, revealing the presence of ventricle- and atrial-like cells. Electrophysiological studies performed on early culture revealed a pacemaker activity of the cells. Finally, functional studies showed that adrenergic agonist stimulated the beating rate whereas cholinergic agonist decreased it. Taken together, this study demonstrated that functional cardiomyocyte-like cells could be directly obtained from adipose tissue. According to the large amount of this tissue in adult mammal, it could represent a useful source of cardiomyocyte progenitors. |
| Starting Page | 31 |
| Ending Page | 39 |
| Page Count | 9 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://circres.ahajournals.org/content/circresaha/94/2/223.full.pdf?download=true |
| Alternate Webpage(s) | http://circres.ahajournals.org/content/circresaha/94/2/223.full.pdf |
| Alternate Webpage(s) | http://cv2i.org/wp-content/uploads/2012/09/Planat-Benard_CR_94_223_2004.pdf |
| PubMed reference number | 14656930v1 |
| Volume Number | 94 |
| Issue Number | 2 |
| Journal | Circulation research |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Adipose tissue Adrenergic Agonists Azacitidine Body tissue Cholinergic Agonists Heart Atrium Immunocytochemistry Mammals Megakaryocyte-Erythroid Progenitor Cells Myocytes, Cardiac Pluripotent Stem Cells Staining method Stroma cardiac muscle cell differentiation |
| Content Type | Text |
| Resource Type | Article |
