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Inhibition of JNK-1 by small interfering RNA induces apoptotic signaling in PC-3 prostate cancer cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Parra, Eduardo Candel |
| Copyright Year | 2012 |
| Abstract | Previous studies have shown that c-Jun-N-terminal kinase-1 (JNK-1) is involved in the transformation of primary fibroblasts and plays a role in tumor cell growth. A number of observations suggest that JNK-1 is a growth promoting factor in prostate cancer cells and blocking its function may induce apoptosis. To test this further, we used a small interfering RNA (siRNA) against JNK-1 mRNA that efficiently inhibits JNK-1 expression in the prostate cancer cell line, PC-3. The application of siRNA against JNK-1 decreased the expression of JNK-1 and affected the expression of p21, XIAP and Bcl-2, but had no effect on the expression of VEGF. In contrast, a control scramble siRNA did not affect the expression of the above indicated proteins. The downregulation of JNK-1 expression at both the mRNA and protein levels was detected by reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis. Cell proliferation inhibition rates were determined by the MTT assay. The effect of JNK-1-siRNA on cell cycle distribution and cell apoptosis was determined by flow cytometry, DNA fragmentation and caspase activity. Our data showed that siRNA against JNK-1 mRNA, could efficiently suppress the expression of JNK-1 in PC-3 cells. After 5 days of transfection, the cell death rate was 52%, the apoptotic rate 26% and the viability rate 22%. In conclusion, downregulation of JNK-1 expression by siRNA against JNK-1 mRNA induces apoptotic signaling in prostate cancer PC-3 cells. The use of siRNA against JNK-1 as a novel approach to cancer therapy deserves further investigation. |
| Starting Page | 113 |
| Ending Page | 120 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.spandidos-publications.com/ijmm/30/4/923/download |
| PubMed reference number | 22766602v1 |
| Alternate Webpage(s) | https://doi.org/10.3892/ijmm.2012.1055 |
| DOI | 10.3892/ijmm.2012.1055 |
| Journal | International journal of molecular medicine |
| Volume Number | 30 |
| Issue Number | 4 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Cell Cycle Cell Death Cell Proliferation Cessation of life Cultured Cell Line DNA Fragmentation Down-Regulation Flow Cytometry Neoplastic Cell Non-Small Cell Lung Carcinoma Promotion (action) Prostatic Neoplasms RNA RNA, Small Interfering Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Western Blotting XIAP gene apoptotic signaling pathway cancer cell cell growth monooxyethylene trimethylolpropane tristearate |
| Content Type | Text |
| Resource Type | Article |
