Loading...
Please wait, while we are loading the content...
Similar Documents
Anti-tumor activity of dendritic cell-cytokine induced killer cells ( DC-CIKs ) sensitized to HER 2 against HER-positive breast cancer cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wen, Yanhua Hu, X. Sylvia |
| Copyright Year | 2017 |
| Abstract | This study aimed to investigate the cytotoxicity of cytokineinduced killer cells (CIKs) and Her2 epitope peptide-sensitized dendritic cells (DCs), when co-cultured with Her2-positive MCF-7 cells. DCs were separated from the Her epitope peptide-sensitized peripheral blood; the Her epitope combines directly with the MHC-II molecule on the DC surface. The DCs were co-cultured with autologous CIKs. Lactate dehydrogenase (LDH) and ELISA kits were used to detect cytotoxicity of CIKs against MCF-7 breast cancer cells; IL-12 and IFN-γ levels were also analyzed in the supernatant of the culture medium. CIKs activated by DCs sensitized by anchored Her polypeptide antigen have greater cytotoxicity against MCF-7 than CIKs alone or non-anchored antigen sensitized DCs-CIKs (P < 0.01); the IL-12 and IFN-γ levels in the supernatant were higher than that of the control (P < 0.01). In conclusion, DCs anchored by polypeptide antigen alone or in combination with effector cells can be used to develop therapeutic DC vaccines against breast cancer. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.funpecrp.com.br/gmr/year2016/vol15-2/pdf/gmr7853.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
