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Comparative investigation of the effects of specific antigen‑sensitized DC‑CIK and DC‑CTL cells against B16 melanoma tumor cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Ren, Peng-Tao Zhang, Yuan |
| Copyright Year | 2017 |
| Abstract | The use of personalized adoptive immunotherapy as a potential novel approach is promising in the treatment of tumors resistant to conventional therapies. In the present study, dendritic cell (DC)-cytokine-induced killer (CIK) and DC-cytotoxic lymphocyte (CTL) cells were cultured to examine their phenotype, proliferation and cytotoxicity against B16 melanoma tumor cells. In addition, comparative investigations of the effect of specific antigen-sensitized DC-CIK and DC-CTL cells against B16 melanoma tumor cells were performed in vitro and in vivo. The results showed that the phenotypes of the co-cultured cells were altered, and DCs promoted DC-CIK cell and DC-CTL cell differentiation and maturation in vitro. Lactate dehydrogenase cytotoxic analysis indicated that the cytotoxicity increased as the effector to target ratio increased between 10:1 and 40:1, and the cytotoxic effect towards B16 melanoma cells by DC-CTL cells was significantly higher, compared with that of DC-CIK cells. To further examine the antineoplastic efficacy of DC-CIK and DC-CTL cells in vivo, the present study performed tail-intravenous injection of DC-CIK cells and DC-CTL cells, which attenuated B16 melanoma cell-engrafted tumor growth, induced G0/G1 cell cycle arrest and accelerated cell apoptosis. Taken together, these results suggested that the use of DC-CTL or DC-CIK cell therapy as a personalized adoptive immunotherapy may regulate immune status and inhibit tumor growth in vivo. In addition, the experiments indicated that DC-CTL cells offer superior antineoplastic activity, compared with DC-CIK cells against B16 melanoma tumor cells. |
| ISSN | 17912997 |
| Journal | Molecular Medicine Reports |
| Volume Number | 15 |
| PubMed Central reference number | PMC5364993 |
| Issue Number | 4 |
| PubMed reference number | 28260039 |
| e-ISSN | 17913004 |
| DOI | 10.3892/mmr.2017.6175 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2017-02-07 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Ren et al. |
| Subject Keyword | dendritic cell-cytokine-induced killer dendritic cell-cytotoxic lymphocyte melanoma immunotherapy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Molecular Biology Biochemistry Cancer Research Molecular Medicine Oncology |
