NDLI: Focused Electrospray Deposition for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

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Focused Electrospray Deposition for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Content Provider	Semantic Scholar
Author	Jeong, Kyung Hwan Seo, Jongcheol Yoon, Hye-Joo Shin, Seung Koo
Copyright Year	2010
Abstract	Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while α-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1‒3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive detection of peptides by homogeneously depositing matrix and sample under atmospheric pressure.
Starting Page	2293
Ending Page	2298
Page Count	6
File Format	PDF HTM / HTML
DOI	10.5012/bkcs.2010.31.8.2293
Volume Number	31
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/chemical/JCGMCS/2010/v31n8/JCGMCS_2010_v31n8_2293.pdf
Alternate Webpage(s)	https://doi.org/10.5012/bkcs.2010.31.8.2293
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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