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Connecting DNA origami structures using the biotin-streptavidin specific binding
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zhou, Ming Anthony, Thalia |
| Copyright Year | 2015 |
| Abstract | This work made use of the strong interaction between biotin and streptavidin to connect designed DNA origami structures. The caDNAno software was used to design a 6 layer 3D origami cross-like structure. Selected DNA strands at the engineered attachment sites on the DNA origami structure were biotinylated. After folding of the origami structures, the biotinylated strands stick out of the attachment sites. Purified samples of origami structures were then mixed with streptavidin and the mixture purified. After characterization, we see that attachment only occurs at the biotinylated sites. Agarose gel electrophoresis, UV-vis spectroscopy and TEM were used to characterize the structure. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.ajol.info/index.php/ajb/article/download/120423/109899 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Attachments Biotin DNA barcoding DNA microarray End-to-end principle Ethanol 0.62 ML/ML Topical Gel Gel Electrophoresis (lab technique) Interaction Sol-gel Sticky bit Streptavidin interest |
| Content Type | Text |
| Resource Type | Article |
