Loading...
Please wait, while we are loading the content...
Similar Documents
Polyacrylamide gel photopatterning enables automated protein immunoblotting in a two-dimensional microdevice.
| Content Provider | Semantic Scholar |
|---|---|
| Author | He, Mei Herr, Amy E. |
| Copyright Year | 2010 |
| Abstract | We demonstrate a two-dimensional microfluidic architecture that integrates polyacrylamide gel electrophoresis (PAGE) with immunoblotting in a fully automated format. This assay is designed to overcome performance limitations of conventional slab-gel immunoblotting, including multiple manual interventions, low-throughput transfer and blotting, and substantial consumption of reagents and sample. To unify PAGE with blotting in one device, this microfluidic approach makes use of high-resolution regional photopatterning of multiple polyacrylamide gel elements, and automated electrophoretic transport. A complete native immunoblot of free prostate specific antigen from human seminal fluid is demonstrated in less than 5 min. Further, the characterization of post-PAGE electrophoretic transfer showed high efficiency and minimal sample dispersion. |
| Starting Page | 151 |
| Ending Page | 151 |
| Page Count | 1 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://herrlab.berkeley.edu/manuscripts/jacs10_he.pdf |
| Alternate Webpage(s) | http://herrlab.berkeley.edu/manuscripts/jacs10_SI_he.pdf |
| PubMed reference number | 20131779v1 |
| Alternate Webpage(s) | https://doi.org/10.1021/ja910164d |
| DOI | 10.1021/ja910164d |
| Journal | Journal of the American Chemical Society |
| Volume Number | 132 |
| Issue Number | 8 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Ethanol 0.62 ML/ML Topical Gel Gel Electrophoresis (lab technique) Immunoblotting Microfluidics Polyacrylamide Gel Electrophoresis Prostate-Specific Antigen Reagents polyacrylamide gels |
| Content Type | Text |
| Resource Type | Article |
