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Mikrofluidische CE-Systeme aus Polymeren mit elektrischer Detektion für Life-Science-Anwendungen
| Content Provider | Semantic Scholar |
|---|---|
| Author | Muehlberger, Holger Hoffmann, Werner Dipl Ing Guber, Andreas E. Saile, Volker |
| Copyright Year | 2007 |
| Abstract | As a contribution to the development of innovative microfluidic lab-on-chip systems, this work presents important results for the practical introduction of capillary electrophoresis (CE) in chip format - in particular with the objective of significant cost reduction. Key contributions are the substitution of conventional substrate glass by polymers and the introduction of contactless conductivity measurement for detection instead of expensive optical methods. In this work CE chips were fabricated and characterized using cost effective polymers such as PMMA, polystyrene and polycarbonate, which are already established in mass production processes, as well as chemically inert and biocompatible high-performance polymers COC and PEEK. By using a new bonding process, PEEK as a near-ideal material, could be used for the first time for microfluidic CE-systems. The principal of contactless conductivity measurement was transferred to chip level and optimized to a high signal-to-noise ratio using sputtered thin film electrodes. Chemical analysis results depend significantly on fluid transport behaviour at the fluid injection cross area. This was investigated by fluorescence microscopy on several different cross geometries. Hydrostatic interfering effects and method based instabilities were shown. As a simple alternative to complex fluorescence microscopy, fluid transport could be optimally observed by conductivity measurement. Measurement electrodes placed at the injection cross area allowed real time quantitative analyses. Thus, interferences and method based instabilities can be acquired and compensated for. For basic experimental investigations, a comprehensive and flexible test setup was developed. In practical application however ease of use, portability and low-priced devices are required. To this end, a completely miniaturized measuring device, the ,,MinCE", was developed. Potential applications were demonstrated on selected typical examples: for in-situ food analysis the determination of organic acids and saccharide in beverages, for medical point-of-care diagnostics the quantitative determination of antidepressant lithium in blood serum and for bio analytics the detection of proteinogenic amino acids. |
| File Format | PDF HTM / HTML |
| DOI | 10.5445/IR/1000006650 |
| Volume Number | 7290 |
| Alternate Webpage(s) | https://publikationen.bibliothek.kit.edu/1000006650/3814962 |
| Alternate Webpage(s) | https://doi.org/10.5445/IR%2F1000006650 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
