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Induction of mossy fiber --> Ca3 long-term potentiation requires translocation of synaptically released Zn2+.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Yang Hough, Christopher J. |
| Copyright Year | 2001 |
| Abstract | The mammalian CNS contains an abundance of chelatable Zn(2+) sequestered in the vesicles of glutamatergic terminals. These vesicles are particularly numerous in hippocampal mossy fiber synapses of the hilar and CA3 regions. Our recent observation of frequency-dependent Zn(2+) release from mossy fiber synaptic terminals and subsequent entry into postsynaptic neurons has prompted us to investigate the role of synaptically released Zn(2+) in the induction of long-term potentiation (LTP) in field CA3 of the hippocampus. The rapid removal of synaptically released Zn(2+) with the membrane-impermeable Zn(2+) chelator CaEDTA (10 mm) blocked induction of NMDA receptor-independent mossy fiber LTP by high-frequency electrical stimulation (HFS) in rat hippocampal slices. Mimicking Zn(2+) release by bath application of Zn(2+) (50-100 microm) without HFS induced a long-lasting potentiation of synaptic transmission that lasted more than 3 hr. Moreover, our experiments indicate the effects of Zn(2+) were not attributable to its interaction with extracellular membrane proteins but required its entry into presynaptic or postsynaptic neurons. Co-released glutamate is also essential for induction of LTP under physiological conditions, in part because it allows Zn(2+) entry into postsynaptic neurons. These results indicate that synaptically released Zn(2+), acting as a second messenger, is necessary for the induction of LTP at mossy fiber-->CA3 synapses of hippocampus. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ohio.edu/people/liy1/Publication%20PDF/Li-LTP2001.pdf |
| Alternate Webpage(s) | http://www.jneurosci.org/content/jneuro/21/20/8015.full.pdf |
| PubMed reference number | 11588174v1 |
| Volume Number | 21 |
| Issue Number | 20 |
| Journal | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | CA3 field Electric stimulation technique Glutamic Acid Greater Than Hyalinosis, Systemic Iron Chelator [EPC] Long-Term Potentiation Mammals Membrane Proteins Mossy Fibers, Hippocampal N-Methyl-D-Aspartate Receptors N-Methylaspartate Presynaptic Terminals Synapses Synaptic Transmission Tissue fiber Tissue membrane Vesicle (morphologic abnormality) chemosensitization/potentiation micacocidin A mossy fiber second messenger zinc cation |
| Content Type | Text |
| Resource Type | Article |
