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The Pattern of Methylation and Polymorphism in Interleukin-6 Promoter Gene Are Related to the Development of Rheumatoid Arthritis?
| Content Provider | Semantic Scholar |
|---|---|
| Author | Gomes-Silva, Isaura Isabelle Fonseca Rushansky, Eliézer Carvalho-Neto, Francisco Geraldo De Vieira, Amanda Virginia Batista Mariano, Maria Helena Souza, Paulo Roberto Eleutério Montes, Martín Alejandro Maia, Maria Mascena |
| Copyright Year | 2018 |
| Abstract | Rheumatoid Arthritis (RA) is a complex disease with higher level of IL-6 . In order to elucidate the alterations related to IL-6 gene in RA, we evaluated the -174 G/C IL-6 gene polymorphism and methylation pattern of its promoter. A total of 120 RA patients and 120 healthy controls were recruited for polymorphism analysis, and 30 individuals of both groups were randomly selected for methylation analysis. The IL-6 gene polymorphism was analyzed by PCR-RFLP and methylation pattern was analyzed by MSP-PCR . Regarding IL-6 gene polymorphism, no significant difference was found between RA patients and controls ( P >0.05). For DNA, two CpG regions of IL-6 promoter gene was analyzed. The first region (−1069, −1061, −1057 and −1001 CpG ) did not show significant differences, whereas the second region (−628, −610, −574 and −491 CpG ) showed a significant hypermethylated status ( P =0.0004) in RA patients as compared with controls.These results, suggest a possible relationship between methylation pattern and the susceptibility to RA in our studied population. |
| Starting Page | 429 |
| Ending Page | 433 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| Volume Number | 56 |
| Alternate Webpage(s) | http://acta.tums.ac.ir/index.php/acta/article/download/6344/5125 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
