NDLI: The Pattern of Methylation and Polymorphism in Interleukin-6 Promoter Gene Are Related to the Development of Rheumatoid Arthritis?

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The Pattern of Methylation and Polymorphism in Interleukin-6 Promoter Gene Are Related to the Development of Rheumatoid Arthritis?

Content Provider	Directory of Open Access Journals (DOAJ)
Author	Isaura Isabelle Fonseca Gomes-Silva Eliezer Rushansky Martín Alejandro Montes Paulo Roberto Eleutério de Souza Amanda Virginia Batista Vieira Maria Helena Queiroz de Araujo Mariano Francisco Geraldo Carvalho-Neto Maria Mascena Diniz Maia
Abstract	Rheumatoid Arthritis (RA) is a complex disease with higher level of IL-6. In order to elucidate the alterations related to IL-6 gene in RA, we evaluated the -174 G/C IL-6 gene polymorphism and methylation pattern of its promoter. A total of 120 RA patients and 120 healthy controls were recruited for polymorphism analysis, and 30 individuals of both groups were randomly selected for methylation analysis. The IL-6 gene polymorphism was analyzed by PCR-RFLP and methylation pattern was analyzed by MSP-PCR. Regarding IL-6 gene polymorphism, no significant difference was found between RA patients and controls (P>0.05). For DNA, two CpG regions of IL-6 promoter gene was analyzed. The first region (−1069, −1061, −1057 and −1001 CpG) did not show significant differences, whereas the second region (−628, −610, −574 and −491 CpG) showed a significant hypermethylated status (P=0.0004) in RA patients as compared with controls.These results, suggest a possible relationship between methylation pattern and the susceptibility to RA in our studied population.
Related Links	https://acta.tums.ac.ir/index.php/acta/article/view/6344
ISSN	00446025
e-ISSN	17359694
Journal	Acta Medica Iranica
Issue Number	7
Volume Number	56
Language	English
Publisher	Tehran University of Medical Sciences
Publisher Date	2018-01-01
Publisher Place	Iran
Access Restriction	Open
Subject Keyword	Medicine (general) Cytokine Inflammation Epigenetics Msp-pcr Single Nucleotide Polymorphism
Content Type	Text
Resource Type	Article
Subject	Medicine

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