NDLI: Penetration of clinical isolates of Pseudomonas aeruginosa through MDCK epithelial cell monolayers.

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Penetration of clinical isolates of Pseudomonas aeruginosa through MDCK epithelial cell monolayers.

Content Provider	Semantic Scholar
Author	Hirakata, Yoichi Kohno, Shigeru Kamihira, Shimeru Speert, David Paul
Copyright Year	2000
Abstract	Pseudomonas aeruginosa causes both invasive (bacteremic) and chronic noninvasive infections. A simple in vitro system to screen P. aeruginosa clinical isolates for their capacity to penetrate MDCK cell monolayers has been developed. By means of this system, P. aeruginosa clinical isolates, including 32 blood and 45 respiratory isolates, were examined. When monolayers were infected with 3.5x107 cfu of bacteria, significantly more blood (93.7%) than respiratory (54.4%) isolates (P<.001) were detected in the basolateral medium after 3 h. Penetration ability was usually independent of cytotoxicity. Only 8 (4 blood and 4 respiratory) isolates were cytotoxic, possessed exoU, and passed through the monolayer after epithelial cell death, associated with a marked drop in transepithelial electrical resistance. Conversely, noncytotoxic isolates with high penetration ability but without severe epithelial damage were invasive. This system is well suited for screening clinical isolates and their mutants for specific genes conferring the invasiveness phenotype.
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PubMed reference number	10669373v1
Volume Number	181
Issue Number	2
Journal	The Journal of infectious diseases
Language	English
Access Restriction	Open
Subject Keyword	Cell Death Cessation of life Colony-forming unit Epithelial Cells Pseudomonas aeruginosa
Content Type	Text
Resource Type	Article

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