NDLI: Construction and characterization of a live attenuated vaccine candidate against Shigella dysenteriae type 1.

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Construction and characterization of a live attenuated vaccine candidate against Shigella dysenteriae type 1.

Content Provider	Semantic Scholar
Author	Klee, S. R. Tzschaschel, B. D. Fält, I. C. Kärnell, Anders Lindberg, A. A. Timmis, Kenneth N. Guzmán, Carlos Alberto
Copyright Year	1997
Abstract	Vaccine candidates against Shigella dysenteriae type 1, which is associated with the most severe cases of bacillary dysentery, were constructed. The rfp and rfb gene clusters, which code for S. dysenteriae 1 O antigen biosynthesis, were randomly integrated into either the chromosome or the virulence plasmid of the rough attenuated Shigella flexneri aroD strain SFL124-27 with a minitransposon carrying an arsenite resistance selection marker. The recombinant clones efficiently expressed the recombinant O antigen, exhibited a normal growth pattern, were able to invade and survive within eukaryotic cells to the same extent as the parental strain, and expressed the recombinant antigen within invaded cells. A clone was selected as the vaccine candidate, which was demonstrated to be immunogenic and safe in animal models, leading to 47% full protection and 53% partial protection against challenge with the wild-type strain.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://hzi.openrepository.com/bitstream/handle/10033/8535/Klee%20et%20al_final.pdf?isAllowed=y&sequence=1
Alternate Webpage(s)	http://iai.asm.org/content/65/6/2112.full.pdf
PubMed reference number	9169740
Volume Number	65
Issue Number	6
Journal	Infection and immunity
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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