NDLI: Comparison of Agar Gel Immunodiffusion Test , Immunohistochemsitry and Reverse Transcription-polymerase Chain Reaction for Detection of Infectious Bursal Disease Virus

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Comparison of Agar Gel Immunodiffusion Test , Immunohistochemsitry and Reverse Transcription-polymerase Chain Reaction for Detection of Infectious Bursal Disease Virus

Content Provider	Semantic Scholar
Author	Islam, Taohidul Khan, Muhammad Imran Islam, Aminul
Copyright Year	2013
Abstract	The objective of this study was to compare agar gel immunodiffusion test (AGID) and immunohistochemistry (IHC) with reverse transcriptase-polymerase chain reaction (RT-PCR) in terms of sensitivity and specificity for the detection of infectious bursal disease virus (IBDV). Thirty-five bursal samples collected from filed outbreak of IBD were evaluated by all 3 diagnostic tests. Sensitivity and specificity of both AGIDT and IHC with RT-PCR was 94.12% and 100%, respectively. Both AGIDT and IHC showed a 94.29% association with RT-PCR with a k value of 0.482, indicating a moderate degree of agreement. The Cochran’s Q value was 4.00, which is lower than the critical value, indicating that the methods did not differ significantly (p>0.05) from each other in detection of samples as positive or negative. However, RT-PCR had distinct edge over these two tests employed in the study.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://pakacademicsearch.com/pdf-files/med/121/121-125%20Vol%209,%20No%202%20(2011).pdf
Language	English
Access Restriction	Open
Subject Keyword	Agar Gel Immunodiffusion Immunodiffusion measurement Immunohistochemistry Infectious bursal disease virus Ion beam deposition Irritable Bowel Syndrome Medical transcription RNA-Directed DNA Polymerase Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Sensitivity and specificity Windows RT
Content Type	Text
Resource Type	Article

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