NDLI: COMPARISON OF AGAR GEL IMMUNODIFFUSION TEST, IMMUNOHISTOCHEMSITRY AND REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

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COMPARISON OF AGAR GEL IMMUNODIFFUSION TEST, IMMUNOHISTOCHEMSITRY AND REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

Content Provider	Scilit
Author	Islam, Mt Islam, Mn Khan, Mzi Islam, Ma
Copyright Year	2013
Description	The objective of this study was to compare agar gel immunodiffusion test (AGID) and immunohistochemistry (IHC) with reverse transcriptase-polymerase chain reaction (RT-PCR) in terms of sensitivity and specificity for the detection of infectious bursal disease virus (IBDV). Thirty-five bursal samples collected from filed outbreak of IBD were evaluated by all 3 diagnostic tests. Sensitivity and specificity of both AGIDT and IHC with RT-PCR was 94.12% and 100%, respectively. Both AGIDT and IHC showed a 94.29% association with RT-PCR with a k value of 0.482, indicating a moderate degree of agreement. The Cochrans Q value was 4.00, which is lower than the critical value, indicating that the methods did not differ significantly (p>0.05) from each other in detection of samples as positive or negative. However, RT-PCR had distinct edge over these two tests employed in the study.DOI: http://dx.doi.org/10.3329/bjvm.v9i2.13452
Ending Page	125
Page Count	5
Starting Page	121
ISSN	17297893
e-ISSN	23080922
DOI	10.3329/bjvm.v9i2.13452
Journal	Bangladesh Journal of Veterinary Medicine
Issue Number	2
Volume Number	9
Language	English
Publisher	Bangladesh Academy of Sciences
Publisher Date	2013-01-22
Access Restriction	Open
Subject Keyword	Disease Prevalence Reverse Transcriptase Pcr Rt Pcr
Content Type	Text
Resource Type	Article

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