Loading...
Please wait, while we are loading the content...
A simple and rapid leaf genomic DNA extraction method for polymerase chain reaction analysis
| Content Provider | Semantic Scholar |
|---|---|
| Author | Amani, Jafar Kazemi, Roohallah Abbasi, Ali Reza Salmanian, Ali Hatef |
| Copyright Year | 2011 |
| Abstract | In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant material to reduce inhibitory agents (alkaloids, phenolic). The procedure involves homogenization of the plant leaf in extraction buffer, incubation at 60oC, extraction by chloroform: iso-amyl alcohol and finally DNA precipitation by cold isopropanol. The results showed that the extracted DNA could be used directly for PCR. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ijbiotech.com/article_7144_37c05c8092c9229060b05f685c0cad0a.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
