A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.

Content Provider	Europe PMC
Author	Jang, Ji Yun Liang, Ting Kim, Myeong-Kyu Kang, Kyung Wook Lee, Bora Choi, Seok-Yong
Copyright Year	2022
Abstract	Genotyping usually entails analysis of the products of polymerase chain reaction (PCR) carried out with genomic DNA (gDNA) as template, and is employed for validation of mutant or transgenic organisms. For genotyping of adult zebrafish, gDNA is often extracted from clipped caudal fin or skin mucus through either alkaline lysis using NaOH or proteinase K (PK) treatment. Further purification of the gDNA using ethanol precipitation was optional. To develop a rapid and noninvasive method that extracts PCR-ready gDNA from adult zebrafish, we combined skin swabbing with PK treatment and demonstrated its efficiency. This method could be applied to a wide range of fish.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9419989&blobtype=pdf
Page Count	5
ISSN	15458547
Volume Number	19
DOI	10.1089/zeb.2022.0015
PubMed Central reference number	PMC9419989
Issue Number	4
PubMed reference number	35877315
Journal	Zebrafish
e-ISSN	15578542
Language	English
Publisher	Mary Ann Liebert, Inc., publishers
Publisher Date	2022-07-26
Publisher Place	USA
Access Restriction	Open
Rights License	This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © Ji Yun Jang et al., 2022; Published by Mary Ann Liebert, Inc.
Subject Keyword	extraction genomic DNA genotyping Q-tips swabbing zebrafish
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Animal Science and Zoology