NDLI: WAP four-disulfide core domain protein 2 mediates the proliferation of human ovarian cancer cells through the regulation of growth- and apoptosis-associated genes.

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WAP four-disulfide core domain protein 2 mediates the proliferation of human ovarian cancer cells through the regulation of growth- and apoptosis-associated genes.

Content Provider	Semantic Scholar
Author	Chen, Yao Mu, Xia Wang, Suihai Zhao, Liang Wu, Yingsong Li, Jiliang Li, Ming
Copyright Year	2013
Abstract	The WAP four-disulfide core domain protein 2 (WFDC2) is frequently overexpressed in epithelial ovarian cancer cells and has been proposed as a potential biomarker. The biological function of WFDC2 in tumor progression remains unclear. In this study, the stable expression of short hairpin RNA (shRNA) against WFDC2 in the human ovarian SKOV3 cell line was established. Cell proliferation in vitro was determined by MTT assay. Cell cycle and apoptosis were analyzed by FACS. The expression of genes related to cell proliferation and survival was detected by real-time RT-PCR and western blotting. In vivo tumor growth assay was performed by establishing WFDC2-knockdown xenografts in nude mice and monitoring tumor growth. The expression of WFDC2, Ki67 and activated caspase-3 was analyzed by immunohistochemistry in order to determine the role of WFDC2 in proliferation and apoptosis. Our results revealed that the silencing of WFDC2 abolished ovarian cancer cell proliferation, suppressing tumor formation and growth in ovarian cancer cells both in vitro and in vivo. The knockdown of WFDC2 induced upregulation of Fasl and the downregulation of cyclin D1 activated caspase-3 and Ki67. These results indicate that WFDC2 plays a crucial role in tumor formation and growth in ovarian cancer cells. WFDC2 may be a potential therapeutic target for epithelial ovarian cancer.
Starting Page	2712
Ending Page	2723
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://www.spandidos-publications.com/or/29/1/288/download
Alternate Webpage(s)	https://pearl.plymouth.ac.uk/bitstream/handle/10026.1/10294/Chen%20Y%20et%20al.,%202013.pdf?isAllowed=y&sequence=1
PubMed reference number	23129262v1
Alternate Webpage(s)	https://doi.org/10.3892/or.2012.2114
DOI	10.3892/or.2012.2114
Journal	Oncology reports
Volume Number	29
Issue Number	1
Language	English
Access Restriction	Open
Subject Keyword	Activated Caspase-3 Apoptosis Biological Markers Cell Cycle Cell Proliferation Down-Regulation Epithelial ovarian cancer Malignant neoplasm of ovary Neoplasms Short Hairpin RNA Tumor Progression Up-Regulation (Physiology) WFDC2 gene WFDC2 protein, human Western Blotting Xenograft procedure cancer cell fluorescence activated cell sorter device monooxyethylene trimethylolpropane tristearate ovarian neoplasm
Content Type	Text
Resource Type	Article

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