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Selection and design of new primers for multiplex polymerase chain reaction (PCR) detection of transgenes in genetically modified (GM) soybean (Glycine max L.) Roundup Ready Soybean GTS 40-3-2
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hedreyda, Cynthia T. Roxas, Jennifer Lising |
| Copyright Year | 2010 |
| Abstract | Detection limits for the 315-bp CamV35S/EPSPS and the 151-bp nos terminator fragments by the multiplex PCR procedure were 0.5% and 0.3%, respectively. New sets of detection primers were designed in this study based on sequences of amplicons from the initial multiplex PCR experiment in order to obtain additional primers that could be used for transgene detection. The alternative multiplex PCR using the new sets of primers and GM soybean Roundup Ready GTS 40-3-2 DNA template, resulted in different sized amplicons for the native soybean lectin gene fragment (430-bp), the CamV promoter/CP4-EPSPS (a modified form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase, 300-bp), and the nos terminator gene (173-bp). The new detection procedure amplified both transgenes in Roundup Ready GTS 40-3-2 soybean seed samples with distinct and clear bands when using at least 1.0% wt/wt GM soybean Roundup Ready GTS 40-3-2. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://philsciletters.org/2010/2010n2.10.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
