NDLI: Cloning and molecular characterization of GroESL heat-shock operon in methylotrophic bacterium Methylovorus Sp. strain SS1 DSM 11726.

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Cloning and molecular characterization of GroESL heat-shock operon in methylotrophic bacterium Methylovorus Sp. strain SS1 DSM 11726.

Content Provider	Semantic Scholar
Author	Eom, Chi Yong Kim, Eungbin Ro, Young Tae Kim, Si Wouk Kim, Young Min
Copyright Year	2005
Abstract	The groESL bicistronic operon of a restricted facultative methylotrophic bacterium Methylovorus sp. strain SS1 DSM 11726 was cloned and characterized. It was found to consist of two ORFs encoding proteins with molecular masses of 11,395 and 57,396 daltons, which showed a high degree of homology to other bacterial GroES and GroEL proteins. The genes were clustered in the transcription order groES-groEL. Northern blot analyses suggested that the groESL operon is transcribed as a bicistronic 2.2-kb mRNA, the steady-state level of which was markedly increased by temperature elevation. Primer extension analysis demonstrated one potential transcription start site preceding the groESL operon, which is located 100 bp upstream of the groES start codon. The transcription start site was preceded by a putative promoter region highly homologous to the consensus sequences of Escherichia coli sigma 32-type heat shock promoter, which functioned under both normal and heat shock conditions in E. coli. Heat shock mRNA was maximally produced by Methylovorus sp. strain SS1 approximately 10 min after increasing the temperature from 30 to 42 degrees C. The groESL operon was also induced by hydrogen peroxide or salt shock.
Starting Page	695
Ending Page	702
Page Count	8
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://ocean.kisti.re.kr/downfile/volume/ksbmb/E1MBB7/2005/v38n6/E1MBB7_2005_v38n6_695.pdf
PubMed reference number	16336785v1
Volume Number	38
Issue Number	6
Journal	Journal of biochemistry and molecular biology
Language	English
Access Restriction	Open
Subject Keyword	Codon, Initiator Fever HSPD1 gene Heat Shock Protein 27 Heat-Shock Proteins 70 Heat-Shock Response Homologous Gene Hydrogen Peroxide Open Reading Frames Operon Peroxides Primer Extension Promoter Regions, Genetic Transcription Initiation Site interferon alfacon-1
Content Type	Text
Resource Type	Article

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