NDLI: Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida.

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Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida.

Content Provider	Semantic Scholar
Author	Inouye, Sumiko Ebina, Y. Nakazawa, Asako Nakazawa, Teruko
Copyright Year	1984
Abstract	The xylABC operon on the TOL plasmid directs the synthesis of enzymes for conversion of toluene to benzoate and is positively controlled by the regulatory gene xylR. In the study here the nucleotide sequence was determined for the regulatory region of this operon. The in vivo transcription initiation site of the operon was determined by S1 nuclease and reverse transcriptase mapping. RNA was prepared from m-methylbenzyl alcohol-induced cells of Pseudomonas putida and Escherichia coli carrying pTN2, a derivative of the TOL plasmid containing the structural and regulatory genes of the entire toluene-degrading pathway. The amount of E. coli mRNA was estimated to be only 10% of that of P. putida mRNA. Consensus sequences of the -10 region (Pribnow box) and the -35 region (RNA polymerase recognition site) in E. coli genes were not found in the region preceding the transcription initiation site, whereas a sequence complementary to the 3' end of the 16S rRNA of Pseudomonas aeruginosa and E. coli existed in front of the predicted start codon of the xylA gene. These results explain the inefficient expression of TOL genes in E. coli.
Starting Page	1695
Ending Page	1708
Page Count	14
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.pnas.org/content/81/6/1688.full.pdf
PubMed reference number	6324212v1
Volume Number	81
Issue Number	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Language	English
Access Restriction	Open
Subject Keyword	Base Sequence Benzoate Codon, Initiator Ethanol Genes, Regulator Nucleotides Operon Pseudomonas aeruginosa Pseudomonas putida RNA RNA-Directed DNA Polymerase Regulatory Sequences, Nucleic Acid Toluene Transcription Initiation Site Transcription, Genetic interferon alfacon-1 nuclease
Content Type	Text
Resource Type	Article

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