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Anti-glycative effects of asiatic acid in human keratinocyte cells
| Content Provider | Semantic Scholar |
|---|---|
| Author | Wang, Zhihong |
| Copyright Year | 2014 |
| Abstract | Background: Human skin keratinocyte (HaCaT) cells served to examine effects of asiatic acid (AA) at 1, 2, 4 and 8 μM against advanced glycative endproduct (AGE)-modified bovine serum albumin (BSA) induced glycative stress. Results: AGE-BSA treatment reduced cell viability; and increased reactive oxygen species, nitric oxide, protein carbonyl, interleukin (IL)-1beta and tumor necrosis factor-alpha levels in HaCaT cells. Yet AA pretreatments decreased these oxidative and inflammatory factors, dose-dependently lowering nitric oxide synthase activity and expression. AGE-BSA raised activity and expression of caspase-3 and caspase-8. AA pretreatments at 2-8 μM decreased activity and expression of these two caspases. AGE-BSA declined collagen I expression, but enhanced matrix metalloproteinase (MMP)-1, MMP-8 and MMP-9 protein expression. AA pretreatments at 2-8 μM maintained collagen I expression, and reduced three MMPs expression. AGE-BSA also up-regulated RAGE (receptor of AGE), p-p38 and p-JNK expression. AA pretreatments at 2-8 μM suppressed RAGE expression, and at 1-8 μM down-regulated p-p38 and p-JNK expression. Conclusion: Asiatic acid, via its anti-glycative activity, could protect skin. Thus, this compound could be developed as an external agent and applied for personalized medicine. |
| File Format | PDF HTM / HTML |
| DOI | 10.7603/s40681-014-0019-9 |
| PubMed reference number | 25520932 |
| Journal | Medline |
| Volume Number | 4 |
| Alternate Webpage(s) | https://www.globalsciencejournals.com/content/pdf/10.7603%2Fs40681-014-0019-9.pdf |
| Alternate Webpage(s) | https://doi.org/10.7603/s40681-014-0019-9 |
| Journal | BioMedicine |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
