NDLI: Effects of Asiatic Acid on Neurite Outgrowth in Neuro-2a Cells

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Effects of Asiatic Acid on Neurite Outgrowth in Neuro-2a Cells

Content Provider	Semantic Scholar
Author	Asiri, Aishah
Copyright Year	2017
Abstract	Recently, medicinal plants from ancient Ayurvedic medicine have provided clues to the discovery of novel therapeutics for various diseases. In Ayurvedic medicine, a common Indian plant, Centella asiatica i s highly regarded as a "rasayana" or nerve tonic. The Centella extract is used to ward off age-related dementia and to increase memory and intell igence. The mechanism by which Centella improves memory and learning and reduces the risk of dementia is unclear. We recently tested the effects of asiatic acid, the main active component of Centella, on neuronal growth. We hypothesized that asiatic acid will promote neuronal growth and neurite network formation. To test this hypothesis, we examined the effects of asiatic acid on neuronal growth in murine neuroblastoma cells, Neuro2a. Neuro2a cells were cultured for 24 hours in DMEM medium containinglO mM glucose and 1 0% FBS in six-well plates at a concentration of 200,000 cells/well. The cells were further cultured for 72 hours in DMEM containing 1 0 mM glucose and with either 1 μM asiatic acid in ethanol or ethanol alone (vehicle). Cells were photographed, and neurite outgrowth quantified using NeuronJ software. The results revealed that asiatic acid treatment significantly increased the percentage of cells bearing neurites as compared to neurons grown in medium alone. In addition, asiatic acid treatment increased neurite extension and combined length of neurites. To investigate the impact of asiatic acid on bioenergetics in Neuro2a cells, we first analyzed the electron transport chain of the mitochondria via respirometry. The respiration rates of Neuro2a cells cultured in medium containing asiatic acid was significantly (p< 0.05) higher than cells grown in
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://thekeep.eiu.edu/cgi/viewcontent.cgi?article=3723&context=theses&httpsredir=1&referer=
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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