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Rapid detection and further characterization of infection with hepatitis B virus variants containing a stop codon in the distal pre-C region.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Julia Su Zhou Tong, Shide Vitvitski, Ludmila Zoulim, Fabien Trépo, Christian |
| Copyright Year | 1990 |
| Abstract | Recently, hepatitis B virus (HBV) replication in the absence of HBe antigenaemia has been attributed to HBV variants with a TAG stop codon in the distal pre-C region associated with one or two point mutations. We describe here a rapid detection method for the diagnosis of such HBeAg-negative HBV variants using selective oligonucleotide hybridization. The entire pre-C region was amplified by the polymerase chain reaction and hybridized under stringent conditions with non-mutated (M0), one (M1) and two (M2) point-mutated oligonucleotide probes. Of the 15 HBeAg-positive (group I) and 20 HBeAg-negative (group II) serum samples studied, 14 samples in group I and one sample in group II hybridized with M0 only and 18 samples in group II hybridized with M1 or M2, or both. The remaining two samples (from groups I and II, respectively) failed to hybridize with any of the three probes. DNA sequencing confirmed mixed distal pre-C sequences in samples hybridizing with more than one probe and also revealed novel mutations in the distal pre-C region of the two samples which failed to hybridize with any of the probes. The latter sample had a +2 frameshift and hence represented a new type of HBeAg-negative HBV variant. This method may therefore prove useful in the diagnosis of infections by HBeAg-negative HBV variants resulting from common mutations in the pre-C region, as well as for the identification of less common variants with novel mutations in the same region. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/71/9/JV0710091993.pdf?accname=guest&checksum=91452087D3F79AFECD52D7CDEE38FCD1&expires=1544236826&id=id |
| Alternate Webpage(s) | http://www.microbiologyresearch.org/docserver/fulltext/jgv/71/9/JV0710091993.pdf?accname=guest&checksum=5F9EFC5F383B3EFA3FEB5DD29088E330&expires=1541586267&id=id |
| PubMed reference number | 2212990v1 |
| Volume Number | 71 |
| Part | 9 |
| Journal | The Journal of general virology |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Codon, Terminator Frameshift Mutation function HBE1 gene Hepatitis B Virus Hepatitis B e Antigens Herpesvirus 1, Cercopithecine Infection LINC01194 gene New type Nucleic Acid Hybridization Oligonucleotide Probes Polymerase Chain Reaction |
| Content Type | Text |
| Resource Type | Article |
