NDLI: Rapid detection and further characterization of infection with hepatitis B virus variants containing a stop codon in the distal preC region

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Rapid detection and further characterization of infection with hepatitis B virus variants containing a stop codon in the distal preC region

Content Provider	Semantic Scholar
Author	Li, Jisu Tong, Shuping Vitvitski, Ludmila Zoulim, Fabien
Copyright Year	2007
Abstract	Recently, hepatitis B virus (HBV) replication in the absence of HBe antigenaemia has been attributed to HBV variants with a TAG stop codon in the distal preC region associated with one or two point mutations. We describe here a rapid detection method for, the diagnosis of such HBeAg-negative HBV variants using selective oligonucleotide hybridization. The entire preC region was amplified by the polymerase chain reaction and hybridized under stringent conditions with non-mutated (M0), one (M 1) and two (M2) pointmutated oligonucleotide probes. Of the 15 HBeAgpositive (group I) and 20 HBeAg-negative (group II) serum samples studied, 14 samples in group I and one sample in group II hybridized with M0 only and 18 samples in group II hybridized with M1 or M2, or both. The remaining two samples (from groups I and II, respectively) failed to hybridize with any of the three probes. DNA sequencing confirmed mixed distal pre-C sequences in samples hybridizing with more than one probe and also revealed novel mutations in the distal pre-C region of the two samples which failed to hybridize with any of the probes. The latter sample had a +2 frameshift and hence represented a new type of HBeAg-negative HBV variant. This method may therefore prove useful in the diagnosis of infections by HBeAg-negative HBV variants resulting from common mutations in the pre-C region, as well as for the identification of less common variants with novel mutations in the same region.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.microbiologyresearch.org/docserver/fulltext/jgv/71/9/JV0710091993.pdf?accname=guest&checksum=FF1C74B36F4149622C21A8AEC6C44E98&expires=1520015214&id=id
Alternate Webpage(s)	https://www.microbiologyresearch.org/docserver/fulltext/jgv/71/9/JV0710091993.pdf?accname=guest&checksum=D82F7D1FD5F9186903F669E947FBBC13&expires=1564712002&id=id
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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