NDLI: Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle.

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Specific delayed-type hypersensitivity responses to ESAT-6 identify tuberculosis-infected cattle.

Content Provider	Semantic Scholar
Author	Costello, Eamon Aggerbeck, Henrik Rosenkrands, Ida Andersen, Peter Hvilshøj
Copyright Year	2003
Abstract	Human and bovine tuberculosis have long been detected by skin testing with purified protein derivative (PPD), a complex mix of partly denatured mycobacterial antigens with suboptimal specificity. In the present study, skin tests based on ESAT-6, a recombinantly produced antigen highly specific for tuberculosis infection, were investigated. Although ESAT-6 was strongly recognized in vitro and induced high levels of gamma interferon, initial investigations demonstrated that higher doses of ESAT-6 than of PPD were needed to induce substantial delayed-type hypersensitivity reactions. Also, the kinetics of the skin test response differed for the two reagents; PPD showed maximal response at 72 h, but the response to ESAT-6 often peaked later at 96 h. Tests based on an optimized strategy (400 micro g of ESAT-6 measured between 72 and 96 h), in cattle infected with Mycobacterium bovis (n = 22) and animals sensitized by exposure to environmental mycobacteria showed ESAT-6 to have a promising diagnostic potential (sensitivity, 82%; specificity, 100%; optimal cutoff, 3 mm), compared with PPD (sensitivity, 86%; specificity, 90%; optimal cutoff, 4 mm). Larger investigations are required to refine cutoff points for any new diagnostic test, but the present results indicate great potential for skin tests based on specific antigens for accurate in vivo diagnosis of tuberculosis.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://jcm.asm.org/content/41/5/1856.full.pdf
PubMed reference number	12734217v1
Volume Number	41
Issue Number	5
Journal	Journal of clinical microbiology
Language	English
Access Restriction	Open
Subject Keyword	Delayed Hypersensitivity Drug Allergy ELECTROSURGICAL DEVICE Genus Mycobacterium Hypersensitivity skin testing Latent Tuberculosis Mycobacterium bovis Purified Protein Derivative of Tuberculin Reagents Recombinant Interferon-gamma Tuberculosis, Bovine Tuberculosis, Pulmonary
Content Type	Text
Resource Type	Article

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