NDLI: Localization of plastid DNA replication on a nucleoid structure.

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Localization of plastid DNA replication on a nucleoid structure.

Content Provider	Semantic Scholar
Author	Nerozzi, Andrea Coleman, Adam R.
Copyright Year	1997
Abstract	Plastids contain multiple copies of the plastid genome that are arranged into discrete aggregates, termed nucleoids. Nucleoid molecular organization and its possible role in ensuring genome continuity have not yet been carefully explored. We examined the relationship between plastid DNA synthesis and nucleoid cytology in the unicellular chrysophyte Ochromonas danica, which is useful for such work because the genomes in each plastid are arranged in a single ring-shaped nucleoid. Immunocytochemical detection of thymidine analog incorporation into replicating DNA revealed that plastid DNA synthesis occurs at several sites along the ring nucleoid simultaneously, and that all plastids of a single cell display similar replication patterns. Plastid DNA replication was observed in G1, S, and G2 phase cells. Pulse-chase-pulse labelling with two different thymidine analogs revealed that new sites are activated as cells progress through the cell cycle while some old sites continue. The double labelling patterns suggest that the individual genomes are arranged consecutively, either singly or in clusters, along the nucleoid perimeter and that the selection of which genome replicates when is a matter of chance. These observations eliminate a number of alternative hypotheses concerning plastid DNA organization, and suggest how cells might maintain a constancy of plastid DNA amount and why plastid genome variants segregate so rapidly during mitosis.
Starting Page	1028
Ending Page	1028
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.amjbot.org/content/84/8/1028.full.pdf
PubMed reference number	21708658v1
Volume Number	84
Issue Number	8
Journal	American journal of botany
Language	English
Access Restriction	Open
Subject Keyword	Analog Cell Cycle Copy (object) Genome Genome, Plastid Mitosis Plastids Thymidine nucleoid plastid DNA replication
Content Type	Text
Resource Type	Article

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