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protein designed for bimodal fl uorescence and magnetic resonance imaging †
| Content Provider | Semantic Scholar |
|---|---|
| Author | Cheng, Qinqin Yuan, Siming Qian, Junchao Zhong, Kai Qianc, Yinfeng Liu, Yangzhong |
| Copyright Year | 2015 |
| Abstract | Multimodal imaging is a highly desirable biomedical application since it can provide complementary information from each imaging modality. We propose a protein engineering-based strategy for the construction of a bimodal probe for fluorescence and magnetic resonance imaging. A recombinant protein was generated by the fusion of a supercharged green fluorescence protein (GFP) with a lanthanide-binding tag (dLBT) that can stably bind two Gd ions. The GFP–dLBT fusion protein showed strong fluorescence and exhibited efficient contrast enhancement in magnetic resonance imaging. This protein probe improves the MR relaxation more efficiently than Gd-DTPA (gadopentetate dimeglumine). The superior cell-penetrating activity of GFP allows the efficient cellular uptake of this fusion protein and it can thus be used as a cellular imaging probe. Dual imaging was conducted in vitro and in mice. This result indicates that the fusion of different functional domains is a feasible approach for making multi-modal imaging agents. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://pubs.rsc.org/en/content/articlepdf/2015/sc/c5sc01925g |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
