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Avaliação do efeito do peptídeo antimicrobiano P34 sobre a formação de biofilmes de bactérias Gram-positivas e biofilme polimicrobiano
| Content Provider | Semantic Scholar |
|---|---|
| Author | Costa, Géssica Aracéli |
| Copyright Year | 2015 |
| Abstract | The antimicrobial peptide P34, produced by Bacillus sp. P34 is a substance that has been explored by having antibacterial and antiviral potential. The objective of this study was to evaluate the antibiofilm potential of this compound compared to biofilms formed by Staphylococcus aureus and Enterococcus faecalis from food. Evaluations were carried out against planktonic and sessile cells, as well as on biofilms formed by these bacteria. The obtained results evidence that the antimicrobial substance has ability to reduce the percentage of adhered microbial cells to the surface, not being verified complete inhibition of the biofilm. Was observed a metabolic inhibition percentage of cells isolated in the biofilm of S. aureus, however, E. faecalis cells performed metabolically stimulated. The combination of antimicrobial peptide P34 with the nisin bacteriocin showed no synergistic effect on cells of S. aureus F4-1, however, when evaluated individually, bacteriostatic effect was observed on this isolated. In the evaluation of this compound effect on the polymicrobial biofilm formed by Salmonella Enteritidis ATCC 13076, Candida tropicalis 72-A and S. aureus F4-1 on stainless steel AISI 304, difference was observed in the cell layout and biofilm architecture, with reduced adhesion of yeast cells. The evaluation of different culture media used, the presence of glucose is presented as a factor that promoted an increment in the formation of Staphylococcus sp biofilms. The obtained results indicate that the substance presents antibiofilm potential for the S. aureus species, and provide information for further studies about polymicrobial biofilms. Master of Science dissertation in Agricultural Environmental Microbiology, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. (127 p.) August, 2015. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.lume.ufrgs.br/bitstream/handle/10183/129897/000977696.pdf?isAllowed=y&sequence=1 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
