NDLI: International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.

Please wait, while we are loading the content...

International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.

Content Provider	Semantic Scholar
Author	Novais, Angela Baquero, Fernando Machado, Elisabete Carina Pinto Cantón, Rafael Peixe, Luísa Coque, Teresa M.
Copyright Year	2010
Abstract	TEM-24 remains one of the most widespread TEM-type extended-spectrum beta-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-DeltaTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly penetrating clones and highly promiscuous plasmids in the spread of antibiotic resistance, and it contributes to the elucidation of the origin and evolution of TEM-2 ESBL derivatives.
Starting Page	E1
Ending Page	E2
Page Count	1
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://aac.asm.org/content/early/2009/12/07/AAC.00959-09.full.pdf
PubMed reference number	19995930v1
Alternate Webpage(s)	https://doi.org/10.1128/AAC.00959-09
DOI	10.1128/aac.00959-09
Journal	Antimicrobial agents and chemotherapy
Volume Number	54
Issue Number	2
Language	English
Access Restriction	Open
Subject Keyword	Antibiotic Resistance, Microbial Biopolymer Sequencing Chloramphenicol Class Contain (action) Electrophoresis, Gel, Pulsed-Field Enterobacteriaceae Gel Electrophoresis (lab technique) Genetics, Population Hybrids Integrons Kanamycin Klebsiella oxytoca Klebsiella pneumoniae Nucleic Acid Hybridization Pasteurella multocida serotype:Prid:Pt:XXX:Nom:Immunoelectrophoresis Plasmids Pneumonia Proteus mirabilis Sulfonamides Tobramycin Trimethoprim beta-Lactamase
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21 (2009)

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21 ▿

Distribution of extended-spectrum beta-lactamases in clinical isolates of Enterobacteriaceae in Vietnam.

Identity gene expression in Proteus mirabilis.

Genetic circularity of the Proteus mirabilis linkage map.

The XerC recombinase of Proteus mirabilis: characterization and interaction with other tyrosine recombinases.

Proteus mirabilis strains of diverse type have IgA protease activity.

Isolation , Identification and Antibiotic Susceptibility of pathogenic Bacteria Isolated from Clinical Samples

Genotyping of New and Old Proteus mirabilis Isolates from Mansoura Hospitals in Egypt by rpoB Sequence Analysis

International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.

Similar Documents

Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21 (2009)

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21 ▿

Distribution of extended-spectrum beta-lactamases in clinical isolates of Enterobacteriaceae in Vietnam.

Identity gene expression in Proteus mirabilis.

Genetic circularity of the Proteus mirabilis linkage map.

The XerC recombinase of Proteus mirabilis: characterization and interaction with other tyrosine recombinases.

Proteus mirabilis strains of diverse type have IgA protease activity.

Isolation , Identification and Antibiotic Susceptibility of pathogenic Bacteria Isolated from Clinical Samples

Genotyping of New and Old Proteus mirabilis Isolates from Mansoura Hospitals in Egypt by rpoB Sequence Analysis

International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21.