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Aggregation-Induced Emission Mapping Live Cell Viscosity with an Aggregation-Induced Emission Fluorogen by Means of Two-Photon Fluorescence Lifetime Imaging
| Content Provider | Semantic Scholar |
|---|---|
| Author | Chen, Sijie Yuning Zeng, Yan Sun, Qiqi Liu, Yang Zhao, Engui Bai, G. Meenambika Qu, Jianan Y. Hao, Jianhua Tang, Ben Zhong |
| Copyright Year | 2015 |
| Abstract | Intracellular viscosity is a crucial parameter that indicates the functioning of cells. In this work, we demonstrate the utility of TPE-Cy, a cell-permeable dye with aggregationinduced emission (AIE) property, in mapping the viscosity inside live cells. Owing to the AIE characteristics, both the fluorescence intensity and lifetime of this dye are increased along with an increase in viscosity. Fluorescence lifetime imaging of live cells stained with TPE-Cy reveals that the lifetime in lipid droplets is much shorter than that from the general cytoplasmic region. The loose packing of the lipids in a lipid droplet results in low viscosity and thus shorter lifetime of TPE-Cy in this region. It demonstrates that the AIE dye could provide good resolution in intracellular viscosity sensing. This is also the first work in which AIE molecules are applied in fluorescence lifetime imaging and intracellular viscosity sensing. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.ece.ust.hk/~eequ/Dr%20Qu's%20Homepage_files/2015-ChemEurJ-1.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
