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Interleukin-21 is a growth and survival factor for human myeloma cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Brenne, Anne-Tove Ro, Torstein Baade Waage, Anders Sundan, Anders Borset, Magne Hjorth-Hansen, Henrik |
| Copyright Year | 2002 |
| Abstract | Interleukin-21 (IL-21) is a recently cloned cytokine with homology to IL-2, IL-4, and IL-15. In this study we examined the effects of IL-21 on human myeloma cells. We found that IL-21 induced proliferation and inhibited apoptosis of the IL-6-dependent human myeloma cell lines ANBL-6, IH-1, and OH-2. The potency of IL-21 was close to that of IL-6 in the OH-2 cell line. Neutralizing antibodies to IL-6 or the IL-6 receptor transducer chain (gp130) did not affect IL-21-induced DNA synthesis, indicating that IL-21-induced proliferation was not mediated through these proteins. Tumor necrosis factor (TNF), another stimulator of myeloma cell growth, up-regulated the expression level of IL-21 receptor (IL-21R), and combinations of TNF and IL-21 gave synergistic effects on myeloma cell proliferation. Furthermore, 4 of 9 purified samples of primary myeloma cells showed a significant increase in DNA synthesis on stimulation of the cells by IL-21. By Western blot analysis, we demonstrated that the intracellular signaling pathways of IL-21 in myeloma cells involved phosphorylation of Jak1, Stat3, and Erk1/2 (p44/42 mitogen-activated protein kinase). IL-21 is a novel growth and survival factor in multiple myeloma and may represent a target for future therapy. |
| Starting Page | 1 |
| Ending Page | 6 |
| Page Count | 6 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://bloodjournal.hematologylibrary.org/content/99/10/3756.full.pdf |
| Alternate Webpage(s) | http://www.bloodjournal.org/content/bloodjournal/99/10/3756.full.pdf?sso-checked=true |
| PubMed reference number | 11986233v1 |
| Volume Number | 99 |
| Issue Number | 10 |
| Journal | Blood |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Apoptosis Biomarkers, Tumor Cell Proliferation Cultured Cell Line DNA Replication HIV Antibodies Homologous Gene Interleukin Receptor Common gamma Subunit Interleukin-6 Mitogen-Activated Protein Kinases Multiple Myeloma Transducers Tumor Necrosis Factors cytokine interleukin-21 receptor |
| Content Type | Text |
| Resource Type | Article |
