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Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods
| Content Provider | Semantic Scholar |
|---|---|
| Author | Zappelini, Lincohn Martone-Rocha, Solange Dropa, Milena Matté, Maria Helena Tiba, Monique Ribeiro Breternitz, Bruna Suellen Razzolini, Maria Tereza Pepe |
| Copyright Year | 2016 |
| Abstract | Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding pattern—identical or similar to Salmonella Enteritidis and Salmonella Typhimurium—and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification. |
| Starting Page | 4828 |
| Ending Page | 4834 |
| Page Count | 7 |
| File Format | PDF HTM / HTML |
| DOI | 10.1007/s11356-016-8109-8 |
| PubMed reference number | 27987122 |
| Journal | Medline |
| Volume Number | 24 |
| Alternate Webpage(s) | http://www.ial.sp.gov.br/resources/editorinplace/ial/2017_4_6/publicacoes_de_2016.pdf |
| Alternate Webpage(s) | https://doi.org/10.1007/s11356-016-8109-8 |
| Journal | Environmental Science and Pollution Research |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
