NDLI: Construction of a genetically engineered and stable strain of degrading gamma-hexachlorocyclohexane and carbendazim by transposon mini-Tn5.

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Construction of a genetically engineered and stable strain of degrading gamma-hexachlorocyclohexane and carbendazim by transposon mini-Tn5.

Content Provider	Semantic Scholar
Author	Xu, Jingliang Qing Li, Shunpeng
Copyright Year	2008
Abstract	The complete dehydrochlorinase gene linA of a gamma-hexachlorocyclohexane (gamma-HCH) degrading strain Sphingomonas sp. BHC-A, containing promoter and Shine-Dalgarno sequence (SD sequence), was amplified by PCR. The linA gene was inserted into NotI-cut transposon vector pUT/mini-Tn5 (Km(r)) to get a novel transposon vector pUT/mini-Tn5-linA. With the helper plasmid RK600, the transposon vector pUT/mini-Tn5-linA was introduced into one carbendazim degrading gram-positive strain Rhodococcus sp. DJL-6 by triparental conjugation and then the dehydrochlorinase gene linA was integrated into the chromosome of Rhodococcus sp. DJL-6 by the transposon mini-Tn5. The selected multifunctional genetically engineered strain DJL-6A could degrade gamma-HCH and carbendazim simultaneously. The dehydrochlorinase activity of DJL-6A was as strong as that of Sphingomonas sp. BHC-A in 0.05 and 5 microg/mL initial gamma-HCH concentration. The linA of the strain DJL-6A was genetically stabile after successive plating DJL-6A for 30 days on nonselective media.
Starting Page	45
Ending Page	50
Page Count	6
File Format	PDF HTM / HTML
PubMed reference number	18338575
Journal	Medline
Volume Number	48
Issue Number	1
Alternate Webpage(s)	http://journals.im.ac.cn/actamicrocn/ch/reader/create_pdf.aspx?file_no=xb2007-0225
Journal	Wei sheng wu xue bao = Acta microbiologica Sinica
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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