NDLI: Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon

Content Provider	Springer Nature Link
Author	Jiang, Jiandong Zhang, Ruifu Li, Rong Gu, Ji Dong Li, Shunpeng
Copyright Year	2006
Abstract	A genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities (105–107 CFU ml−1), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg kg−1 MP and 25 mg kg−1 carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment.
Starting Page	403
Ending Page	412
Page Count	10
File Format	PDF
ISSN	09239820
Journal	Biodegradation
Volume Number	18
Issue Number	4
e-ISSN	15729729
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-11-08
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biodegradation Carbofuran Genetically engineered microorganism Methyl parathion Mini-transposon Waste Management/Waste Technology Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution Terrestrial Pollution Geochemistry Soil Science & Conservation
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Pollution Environmental Engineering Bioengineering Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Interfacial interaction between methyl parathion-degrading bacteria and minerals is important in biodegradation

Isolation of a methyl parathion-degrading strain Stenotrophomonas sp. SMSP-1 and cloning of the ophc2 gene

Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12

Genetic surface-display of methyl parathion hydrolase on Yarrowia lipolytica for removal of methyl parathion in water

Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Biodegradation of plasticizers by Rhodococcus rhodochrous

Biodegradation of lignocellulosic waste by Aspergillus terreus

Biodegradation kinetics of trans-4-methyl-1-cyclohexane carboxylic acid

Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon

Similar Documents

Interfacial interaction between methyl parathion-degrading bacteria and minerals is important in biodegradation

Isolation of a methyl parathion-degrading strain Stenotrophomonas sp. SMSP-1 and cloning of the ophc2 gene

Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12

Genetic surface-display of methyl parathion hydrolase on Yarrowia lipolytica for removal of methyl parathion in water

Overexpression of methyl parathion hydrolase and its application in detoxification of organophosphates

Removal of methyl parathion and tetrachlorvinphos by a bacterial consortium immobilized on tezontle-packed up-flow reactor

Biodegradation of plasticizers by Rhodococcus rhodochrous

Biodegradation of lignocellulosic waste by Aspergillus terreus

Biodegradation kinetics of trans-4-methyl-1-cyclohexane carboxylic acid

Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon