Loading...
Please wait, while we are loading the content...
A colorimetric biosensor for detection of attomolar microRNA with a functional nucleic acid-based amplification machine.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Dandan Cheng, Wei Yan, Yurong Zhang, Ye Yin, Yibing Ju, Huangxian Ding, Shijia |
| Copyright Year | 2016 |
| Abstract | A functional nucleic acid-based amplification machine was designed for simple and label-free ultrasensitive colorimetric biosensing of microRNA (miRNA). The amplification machine was composed of a complex of trigger template and C-rich DNA modified molecular beacon (MB) and G-rich DNA (GDNA) as the probe, polymerase and nicking enzyme, and a dumbbell-shaped amplification template. The presence of target miRNA triggered MB mediated strand displacement to cyclically release nicking triggers, which led to a toehold initiated rolling circle amplification to produce large amounts of GDNAs. The formed GDNAs could stack with hemin to form G-quadruplex/hemin DNAzyme, a well-known horseradish peroxidase (HRP) mimic, for catalyzing a colorimetric reaction. The modified MB improved the stringent target recognition and reduced background signal. The proposed sensing strategy showed very high sensitivity and selectivity with a wide dynamic range from 10 aM to 1.0 nM, and enabled successful visual analysis of trace amount of miRNA in real sample by the naked eye. This rapid and highly efficient signal amplification strategy provided a simple and sensitive platform for miRNA detection. It would be a versatile and powerful tool for clinical molecular diagnostics. |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/j.talanta.2015.09.010 |
| PubMed reference number | 26695292 |
| Journal | Medline |
| Volume Number | 146 |
| Alternate Webpage(s) | http://sklac.nju.edu.cn/hxju/lunwenlunzhu/paper2016/526%20Talanta%20Li%20DD%202016.pdf |
| Alternate Webpage(s) | https://doi.org/10.1016/j.talanta.2015.09.010 |
| Journal | Talanta |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
