NDLI: HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization in 130 Breast Cancers Using Tissue Microarray and Clinical Follow-up Studies

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HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization in 130 Breast Cancers Using Tissue Microarray and Clinical Follow-up Studies

Content Provider	Semantic Scholar
Author	Chang, Eundeok Lee, Anhi Lee, Eunjung Lee, Hekyung Shin, Okran Oh, Sejeong Kang, Changsuk
Copyright Year	2004
Abstract	Determining of HER-2/neu oncogene amplification has become clinically important for managing breast cancer. Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are currently regarded as the standard methods. Chromogenic in situ hybridization (CISH) was investigated as a new modification with an accurate, sensitive technique. From 1998 to 2002, using CISH and IHC, the amplification and protein expression of the HER-2/neu oncogene were examined using paraffin sections in 130 breast carcinomas and to determine the prognostic role of HER-2/neu for outcome after a follow-up of 24- 64 months. Amplifications by CISH and overexpression by IHC were observed in 28 (22%) and 27 cases (20.8%), respectively. Of the 104 patients, 20 patients (19.2%) with amplification had a shorter disease-free interval (34.9 months vs. 38.0 months in controls) (p=0.372). 15 patients (14.4%) had a disease recurrence, but there is no significant difference between 3 patients amplifying the oncogene and 12 patients without oncogene (20.6 months vs. 19.6 months) (p=0.862). 6 patients (5.8%) of these died. CISH is a useful alternative, particularly for confirming the IHC results. There is no relationship between the early recurrence and the HER-2/neu positive group, but lymph node status was statistically significant.
Starting Page	390
Ending Page	396
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	15201505v1
Volume Number	19
Journal	Journal of Korean medical science
Alternate Webpage(s)	https://pdfs.semanticscholar.org/03ec/c65bbb74842d9ae0b35450b2835ef4705b7d.pdf
Language	English
Access Restriction	Open
Subject Keyword	Anatomic Node Breast Carcinoma Cessation of life Chromogenic in situ Hybridization ERBB2 protein, human Fluorescent in Situ Hybridization Gene Amplification Technique Immunohistochemistry Lymph Node Tissue Malignant neoplasm of breast Nucleic Acid Hybridization Oncogene ErbB2 Oncogenes Patients Tissue Microarray lymph nodes protein expression
Content Type	Text
Resource Type	Article

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