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Her-2/neu Oncogene Amplification by Fluorescence In Situ Hybridization and Protein Overexpression on Immunohistochemistry in Breast Cancer.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lateef, Fouzia Jamal, Saba Nasir, Surrayya |
| Copyright Year | 2018 |
| Abstract | OBJECTIVE To investigate the concordance and discordance between the test results of Her-2/neu by immunohisto-chemistry (IHC) and flourescence In Situ hybridization (FISH) in breast cancer cases. STUDY DESIGN Descriptive cross-sectional study. PLACE AND DURATION OF STUDY Department of Histopathology, Dr. Ziauddin Hospital, Karachi, from 2011 to 2016. METHODOLOGY Forty-three specimens of invasive ductal carcinoma of breast were evaluated for grade and Her-2/neu status using IHC and FISH methods. Concordance and discordance between their results was determined. RESULTS There is 100% concordance between FISH and IHC in cases scoring 0, 1+ (negative) and 3+ (positive) immunostaining. Tumour cases scoring 2+ immunostaining showed amplification in 69.2% cases. All grade-I tumours were non-amplified on FISH, while most of the grade-III tumours showed Her-2/neu amplification on FISH. There is significant association of Her-2/neu IHC with tumour grade and FISH (p<0.05). A fairly high proportion i.e. 69.7% of cases showed Her-2/neu gene amplification. There was high concordance between Her-2/neu testing on IHC and FISH, (Kappa co-efficient 0.466, p <0.001). CONCLUSION Her-2/neu amplification increases with increasing grade of breast cancer. A high proportion of Her-2/neu gene amplified cases indicates aggressive disease in that area and need for FISH testing on large scale, which is the gold standard for equivocal cases on immunohistochemistry. |
| Starting Page | 581 |
| Ending Page | 585 |
| Page Count | 5 |
| File Format | PDF HTM / HTML |
| DOI | 10.29271/jcpsp.2018.08.581 |
| PubMed reference number | 30060783 |
| Journal | Medline |
| Volume Number | 28 |
| Issue Number | 8 |
| Alternate Webpage(s) | https://www.jcpsp.pk/archive/2018/Aug2018/02.pdf |
| Alternate Webpage(s) | https://doi.org/10.29271/jcpsp.2018.08.581 |
| Journal | Journal of the College of Physicians and Surgeons--Pakistan : JCPSP |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
